| # | Eastern Conference | PTS | MP | W | D | L | GS | GC | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Louisville City | 72 | 34 | 22 | 6 | 6 | 65 | 28 | WWDWL |
| 2 |  Memphis 901 | 68 | 34 | 21 | 5 | 8 | 67 | 33 | DWWWL |
| 3 |  Tampa Bay Rowdies | 67 | 34 | 20 | 7 | 7 | 73 | 33 | WWWWL |
| 4 |  Birmingham Legion | 58 | 34 | 17 | 7 | 10 | 56 | 37 | DLLWD |
| 5 |  Pittsburgh Riverhounds | 57 | 34 | 16 | 9 | 9 | 50 | 38 | LDWDD |
| 6 |  Miami FC | 55 | 34 | 15 | 10 | 9 | 47 | 32 | DWLWL |
| 7 |  Detroit City | 54 | 34 | 14 | 12 | 8 | 44 | 30 | DWLLL |
| 8 |  Tulsa Roughnecks | 42 | 34 | 12 | 6 | 16 | 48 | 58 | LDWWL |
| 9 |  Indy Eleven | 41 | 34 | 12 | 5 | 17 | 41 | 55 | WWLWL |
| 10 |  Hartford Athletic | 36 | 34 | 10 | 6 | 18 | 47 | 57 | WLWLL |
| 11 |  Loudoun United | 28 | 34 | 8 | 4 | 22 | 36 | 74 | WLLLD |
| 12 |  Charleston Battery | 25 | 34 | 6 | 7 | 21 | 41 | 77 | LLLLD |
| 13 |  Atlanta United II | 23 | 34 | 6 | 5 | 23 | 39 | 85 | LDLWL |
| 14 | New York RB II | 15 | 34 | 3 | 6 | 25 | 24 | 76 | LDLLL |
| # | Western Conference | PTS | MP | W | D | L | GS | GC | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1 |  San Antonio | 77 | 34 | 24 | 5 | 5 | 54 | 26 | WDWWW |
| 2 |  San Diego Loyal | 60 | 34 | 18 | 6 | 10 | 68 | 55 | WDDLL |
| 3 |  Colorado Springs | 55 | 34 | 17 | 4 | 13 | 59 | 53 | WLWWL |
| 4 |  Sacramento Republic | 53 | 34 | 15 | 8 | 11 | 48 | 34 | LDWWL |
| 5 |  New Mexico United | 51 | 34 | 13 | 12 | 9 | 49 | 40 | DDDWL |
| 6 |  Rio Grande Valley | 49 | 34 | 14 | 7 | 13 | 51 | 40 | LDWWL |
| 7 |  Oakland Roots | 46 | 34 | 11 | 13 | 10 | 51 | 46 | WWLWL |
| 8 |  El Paso Locomotive | 46 | 34 | 13 | 7 | 14 | 56 | 52 | LWLWL |
| 9 |  Las Vegas Lights | 45 | 34 | 12 | 9 | 13 | 40 | 50 | LWDWD |
| 10 |  Phoenix Rising | 42 | 34 | 12 | 6 | 16 | 50 | 58 | DWWLW |
| 11 |  LA Galaxy II | 40 | 34 | 11 | 7 | 16 | 53 | 63 | LWDLD |
| 12 |  Monterey Bay | 40 | 34 | 12 | 4 | 18 | 42 | 59 | DLLLL |
| 13 |  Orange County SC | 34 | 34 | 7 | 13 | 14 | 49 | 59 | LDDLD |
